The authors show through EMSA with recombinant His6-RdgB protein that it binds three of the promoters of the ctv operons and the pnlA promoter. Using DNAse footprinting, they further identify the binding site for RdgB in these promoters. The use RT-PCR to show that RpgB is directly affecting the transcription of orf1a, orf4, rdgB, and pnl.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|