RNA-seq was performed on overproducing HrpB strain. HrpB-like sites were identified through multiple sequence alignment of promoters. BPSS1623, BPSS1621, BPSS1614, and BPSS1592 induction in HrpB overproducing strain was verified by RT-PCR. Site directed mutagenesis was performed on the BPSS1606 promoter and its HrpB dependent activity assessed through beta-gal assays.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|