Curation Information

Joint transcriptional control of xpsR, the unusual signal integrator of the Ralstonia solanacearum virulence gene regulatory network, by a response regulator and a LysR-type transcriptional activator.;Huang J, Yindeeyoungyeon W, Garg RP, Denny TP, Schell MA;Journal of bacteriology 1998 May; 180(10):2736-43 [9573161]
PhcA [P52698, view regulon]
Reported TF sp.
Ralstonia solanacearum AW
Reported site sp.
Ralstonia solanacearum AW
Created by
Erill Lab
Curation notes

Experimental Process

Various lengths of xpsR promoter fragment were tested for activity with beta-gal assays using a phcA mutant. Mutations were introduced to the putative binding site, determined by visual inspection and previous reports, and tested with beta-gal assays. EMSAs with w-t and mutated-site fragments confirmed PhcA binding.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... RS_RS21960 RS_RS21955 RS_RS21950 RS_RS21965
Experimental technique details Beta-gal reporter assay - Experimental technique details EMSA (ECO:0001807) - Experimental technique details Site directed mutagenesis (ECO:0005667) - Experimental technique details Visual sequence inspection (nan) - activator not specified