Binding of PhcA to the xpsR and prhIR promoters was demonstrated with EMSA. The specific binding site in the prhIR promoter was determined with EMSA using varied-length fragments of the promoter. Using a phcA deletion mutant, beta-gal assays with varied-length fragments of the prhIR promoter were assessed for expression. The putative binding site of PhcA in prhIR was determined through visual inspection of the fragments that bound PhcA and that deregulated expression. The putative binding site for the xpsR promoter had been reported previously (PMID: 7868600).
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|