To determine whether candidate effector genes were regulated by hrpB the expression level of the Cya fusion protein between the constitutive-hrpB and delta-hrpB strains was compared through an in vitro adenylate cyclase activity assay.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|