Curation Information

Publication
Identification of novel Ralstonia solanacearum type III effector proteins through translocation analysis of hrpB-regulated gene products.;Mukaihara T, Tamura N;Microbiology (Reading, England) 2009 Jul; 155(Pt 7):2235-44 [19406897]
TF
HrpB [P31778, view regulon]
Reported TF sp.
Ralstonia solanacearum GMI1000
Reported site sp.
Ralstonia solanacearum GMI1000
Created by
Erill Lab
Curation notes
-

Experimental Process

To determine whether candidate effector genes were regulated by hrpB the expression level of the Cya fusion protein between the constitutive-hrpB and delta-hrpB strains was compared through an in vitro adenylate cyclase activity assay.

Transcription Factor Binding Sites


TTCGCCCCCGGCCCCATCCATTCG
TTCGGCTCCGGCCTCATCACTTCG
TTCGCTTTTGGATGCGGCGCTTCG
TTCGCCCATGGAACCGGCGCTTCG
TTCGCCCCCGGCCCCATCCATTCG
TTCGGCTCCGGCCTCATCACTTCG
TTCGCTTTTGGATGCGGCGCTTCG
TTCGCCCATGGAACCGGCGCTTCG

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
TTCGCCCCCGGCCCCATCCATTCG RS_RS22570,
... ... RS_RS22570 RS_RS22565 RS_RS22560 RS_RS22555
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details Cya fusion reporter (ECO:0006002) - activator not specified
TTCGGCTCCGGCCTCATCACTTCG RS_RS21175,
... ... RS_RS21175 RS_RS21170
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details Cya fusion reporter (ECO:0006002) - activator not specified
TTCGCTTTTGGATGCGGCGCTTCG RS_RS21170
... ... RS_RS21170
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details Cya fusion reporter (ECO:0006002) - activator not specified
TTCGCCCATGGAACCGGCGCTTCG RS_RS18580,
... ... RS_RS18580 RS_RS18585 RS_RS18590
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details Cya fusion reporter (ECO:0006002) - activator not specified