Visual inspection revealed a palindromic sequence resembling the known CsoR-binding motif upstream of the copZ-csoR-copA operon. The GGCG-TACCCCACCCCACCTGGGGTGGGGTA-GGAG fragment containing this palindrome was tested for CsoR-binding via DNA affinity purification on the streptavidin surface of a sensor chip. Repression by CsoR, and dependence on copper, of the copZ... operon was demonstrated by in vitro transcription of its cloned promoter. Copper- and csoR-dependence of copZ... operon was established in vivo by DNA array analysis using w-t and csoR mutants in the absence/presence of 1.25 mM CuSO4.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|
|TACCCCACCCCACCTGGGGTGGGGTA||TTHA1718, TTHA1719, TTHA1720,||