Curation Information

Structural and functional characterization of the transcriptional repressor CsoR from Thermus thermophilus HB8.;Sakamoto K, Agari Y, Agari K, Kuramitsu S, Shinkai A;Microbiology (Reading, England) 2010 Jul; 156(Pt 7):1993-2005 [20395270]
CsoR [Q5SHL1, view regulon]
Reported TF sp.
Thermus thermophilus HB8
Reported site sp.
Thermus thermophilus HB8
Created by
Erill Lab
Curation notes
External databases
NCBI GEO (GSE19521) Protein Data Bank (3AAI) .

Experimental Process

Visual inspection revealed a palindromic sequence resembling the known CsoR-binding motif upstream of the copZ-csoR-copA operon. The GGCG-TACCCCACCCCACCTGGGGTGGGGTA-GGAG fragment containing this palindrome was tested for CsoR-binding via DNA affinity purification on the streptavidin surface of a sensor chip. Repression by CsoR, and dependence on copper, of the copZ... operon was demonstrated by in vitro transcription of its cloned promoter. Copper- and csoR-dependence of copZ... operon was established in vivo by DNA array analysis using w-t and csoR mutants in the absence/presence of 1.25 mM CuSO4.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... TTHA1718 TTHA1719 TTHA1720 TTHA1721 TTHA1722 TTHA1723 TTHA1724 TTHA1726 TTHA1727 TTHA1728
Experimental technique details DNA affinity purification (ECO:0005629) - Experimental technique details DNA-array expression analysis (ECO:0005525) - Experimental technique details In-vitro transcription (ECO:0001204) - Experimental technique details Visual sequence inspection (nan) - repressor tetramer