Curation Information

Characterization of Xanthomonas axonopodis pv. citri LexA: recognition of the LexA binding site.;Yang MK, Yang YC, Hsu CH;Molecular genetics and genomics : MGG 2002 Dec; 268(4):477-87 [12471445]
LexA [P60511, view regulon]
Reported TF sp.
Xanthomonas axonopodis pv. citri XW47
Reported site sp.
Xanthomonas axonopodis pv. citri XW47
Created by
Erill Lab
Curation notes

Experimental Process

Northern blot evidences induction of lexA with mit-C. DNAse footprinting identifies palindrome. EMSA is used to test that both halves are necessary. Mutagenesis of half-sites + EMSA drives the point home. More detailed mutagenesis proves that lexA binding and induction are dependent on identified site.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... lexA aarF
Experimental technique details DNAse footprinting (ECO:0005631) - Experimental technique details EMSA (ECO:0001807) - Experimental technique details Luciferase reporter assay (ECO:0005648) - Experimental technique details Northern blot (ECO:0005653) - Experimental technique details Site directed mutagenesis (ECO:0005667) - repressor dimer