The CueR promoter binding sites were determined from Salmonella and E.coli by DNase 1 footprinting. Affinity of CueR for the copA promoter was determined by EMSA. Binding of CueR to the copa promoter was also shown by DNase 1 footprinting. Western blot analysis showed expression of CueR in the presence of certain metals. MEME searches were performed to search for binding motifs.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|