The promoter of lrp was mapped with primer extension. Regulation by Lrp was shown by plrp-lacZ reporter assays comparing w-t and lrp mutant, using truncations and site-directed mutagenesis of the promoter region containing putative sites. Binding was shown first with EMSA on truncated plrp fragments and then confirmed with DNAse footprinting.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|