Curation Information

A new regulatory DNA motif of the gamma subclass Proteobacteria: identification of the LexA protein binding site of the plant pathogen Xylella fastidiosa.;Campoy S, Mazón G, Fernández de Henestrosa AR, Llagostera M, Monteiro PB, Barbé J;Microbiology (Reading, England) 2002 Nov; 148(Pt 11):3583-97 [12427949]
LexA [Q9PH24, view regulon]
Reported TF sp.
Xylella fastidiosa 9a5c
Reported site sp.
Xylella fastidiosa 9a5c
Created by
Erill Lab
Curation notes

Experimental Process

EMSA to prove binding, RT-PCR to show mit-C induction. Serial single-position mutagenesis + EMSA to define binding site

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... XF0122 recA XF0121
Experimental technique details EMSA (ECO:0001807) - Experimental technique details qRT-PCR [RNA] (ECO:0001808) - Experimental technique details Site directed mutagenesis (ECO:0005667) - repressor dimer