Curation Information

Transcriptional regulation of sdiA by cAMP-receptor protein, LeuO, and environmental signals in Salmonella enterica serovar Typhimurium.;Turnbull AL, Kim W, Surette MG;Canadian journal of microbiology 2012 Jan; 58(1):10-22 [22149171]
LeuO [P46924, view regulon]
Reported TF sp.
Salmonella enterica subsp. enterica serovar Typhimurium str. 14028S
Reported site sp.
Salmonella enterica subsp. enterica serovar Typhimurium str. 14028S
Created by
Dinara Sagitova
Curation notes

Experimental Process

sdiA expression was shown to be reduced by 30% in leuO deletion mutant compared to the wild-type strain. EMSA confirmed that LeuO regulated sdiA expression directly. Footprinting further confirmed the EMSA results by showing that LeuO bound to two sites which overlap with the -35 and -10 sequences in the sdiA promoter.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type