sdiA expression was shown to be reduced by 30% in leuO deletion mutant compared to the wild-type strain. EMSA confirmed that LeuO regulated sdiA expression directly. Footprinting further confirmed the EMSA results by showing that LeuO bound to two sites which overlap with the -35 and -10 sequences in the sdiA promoter.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|