A spx:bgaB fusion assay demonstrated repression of spx by YodB. Primer extension and In vitro transcription assays validated this claim. Site directed mutagenesis of the upstream region of spx resulted in derepression. DNase I footprinting localized the binding site. Site directed mutagenesis of this identified site confirmed its role in repression.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|