S1 nuclease experiments identified that zur regulates the expression of rpmG2p, rpmB2p, rpmF2p, and znuA. EMSA confirmed that Zur bound specifically to the promoter regions of these genes. DNase I footprinting determined the sequences bound by Zur in each promoter. Multiple sequence alignment of there identified Zur binding sites revealed homology among them.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|
|TTGACAACGGTTTCCATATTTGTTGAAAATGAGTGTCATGAACGTACG||SCO2505, SCO2506, SCO2507,||