Curation Information

The mntH gene encodes the major Mn(2+) transporter in Bradyrhizobium japonicum and is regulated by manganese via the Fur protein.;Hohle TH, O'Brian MR;Molecular microbiology 2009 Apr; 72(2):399-409 [19298371]
Fur [H7C6Q1, view regulon]
Reported TF sp.
Bradyrhizobium diazoefficiens USDA 110
Reported site sp.
Bradyrhizobium diazoefficiens USDA 110
Created by
Joe Sparenberg
Curation notes

Experimental Process

qPCR showed that mntH expression was repressed when cells were grown in high manganese media and this repression was abolished in low manganese media. qPCR analysis further showed that Fur represses mntH expression in the presence of manganese ions. EMSA confirmed that Fur directly regulates mntH. DNase I footprinting identified that Fur bound in the -56 to -22 region relative to the transcription start site of the mntH. The authors further report that the sequence bound by Fur in the mntH promoter contains three imperfect direct repeat hexamers. Mutations in the third hexamer abolished Fur binding.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... mntH blr5045
Experimental technique details DNAse footprinting (ECO:0005631) - Experimental technique details EMSA (ECO:0001807) - Experimental technique details qPCR [quantitative real-time] (ECO:0005660) - Experimental technique details Site directed mutagenesis (ECO:0005667) - repressor not specified