2D PAGE determined that apo-Fur represses sodB and the results were confirmed by MALDI-TOF. EMSA showed that Fur directly binds to sodB with low affinity. DNase I footprinting showed the sequence of the binding site.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|