qRT-PCR demonstrated activation of glgP1 expression by NrrA. Activation of hetR by NrrA had been identified in previous studies. EMSA confirmed binding of NrrA to the glgP1 and hetR promoter regions. Multiple sequence alignment of glgP1 promoters from Anabaena spp. and Nostoc spp. identified a conserved NrrA binding motif. EMSA showed that deletion of this conserved sequence in the glgP promoter abolished NrrA binding.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|