S1 nuclease mapping experiment showed that catC and furA genes are cotranscribed. Alignment of the furA promoter region with a furS promoter from S.reticuli identified a conserved promoter element. Repression of furA-catC by FurA was verified by Western blot analysis. Deletion analysis of the furA promoter in conjunction with EMSA validated that FurA represses furA-catC directly and delimited the binding region to −79 and −59 relative to the furA start codon.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|