Curation Information

Regulation of the furA and catC operon, encoding a ferric uptake regulator homologue and catalase-peroxidase, respectively, in Streptomyces coelicolor A3(2).;Hahn JS, Oh SY, Roe JH;Journal of bacteriology 2000 Jul; 182(13):3767-74 [10850993]
FurA [Q7BR72, view regulon]
Reported TF sp.
Streptomyces coelicolor A3(2)
Reported site sp.
Streptomyces coelicolor A3(2)
Created by
Dinara Sagitova
Curation notes

Experimental Process

S1 nuclease mapping experiment showed that catC and furA genes are cotranscribed. Alignment of the furA promoter region with a furS promoter from S.reticuli identified a conserved promoter element. Repression of furA-catC by FurA was verified by Western blot analysis. Deletion analysis of the furA promoter in conjunction with EMSA validated that FurA represses furA-catC directly and delimited the binding region to −79 and −59 relative to the furA start codon.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... SCO0561 SCO0560 SCO0559 SCO0558 SCO0562
Experimental technique details EMSA (ECO:0001807) - Experimental technique details Multiple sequence alignment (MSA) (ECO:0005556) - Experimental technique details S1 nuclease protection (ECO:0005666) - Experimental technique details Western blot (quantitative) expression analysis (ECO:0000279) - repressor not specified