Curation Information

FeON-FeOFF: the Helicobacter pylori Fur regulator commutates iron-responsive transcription by discriminative readout of opposed DNA grooves.;Agriesti F, Roncarati D, Musiani F, Del Campo C, Iurlaro M, Sparla F, Ciurli S, Danielli A, Scarlato V;Nucleic acids research 2014 Mar; 42(5):3138-51 [24322295]
Fur [B5Z6G7, view regulon]
Reported TF sp.
Helicobacter pylori G27
Reported site sp.
Helicobacter pylori G27
Created by
Joe Sparenberg
Curation notes

Experimental Process

EMSA determined that Fur binds to the OPI(frpB) promoter. Increasing Fur concentration showed a 10-fold decrease in Fur affinity to the OPI(frpB) operator. Multiple Sequence alignment of Fur binding sites identified a consensus sequence. •OH footprinting determined the binding sequence in the OPI(frpB) promoter.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... HPG27_830 HPG27_829 HPG27_828 HPG27_827
Experimental technique details Beta-gal reporter assay - Experimental technique details Consensus search (ECO:0005624) - Experimental technique details EMSA (ECO:0001807) - Experimental technique details Hydroxyl-radical footprinting (ECO:0005643) - Experimental technique details Multiple sequence alignment (MSA) (ECO:0005556) - repressor dimer