Curation Information

Acid-induced activation of the urease promoters is mediated directly by the ArsRS two-component system of Helicobacter pylori.;Pflock M, Kennard S, Delany I, Scarlato V, Beier D;Infection and immunity 2005 Oct; 73(10):6437-45 [16177315]
ArsR [O24973, view regulon]
Reported TF sp.
Helicobactor Pylori 26695
Reported site sp.
Helicobacter pylori G27
Created by
Elizabeth Cardosa
Curation notes

Experimental Process

Primer extension experiments were performed on RNAs extracted from mutant and non-mutant strains of helicobactor pylori G27 to identify that ureA and ureI are regulated by ArsR. DNase footprinting results show that there is direct binding of ArsR~P. Primer extension assay and western blot were used to show expression of the urease genes.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type