Primer extension experiments were performed on RNAs extracted from mutant and non-mutant strains of helicobactor pylori G27 to identify that ureA and ureI are regulated by ArsR. DNase footprinting results show that there is direct binding of ArsR~P. Primer extension assay and western blot were used to show expression of the urease genes.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|