Site directed mutagenesis of the C-terminal end of TraR abolished transcription of traI and traM. Results were supported by lacZ analyses. EMSA confirmed that TraR binds to tra box II and tra box IV. Western blot analyses quantified TraR accumulations of the various mutants in vivo.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|