Using EMSAs, the authors established the boundaries of the operator region for the relBE operon. Serial mutations were introduced to the operator region and the results analyzed by beta-gal fusions. This identified two palindromes bound by two RelB (or RelBE) dimers, and insertion mutagenesis confirmed that their specific spacing is critical for binding of RelBE. These contacts were confirmed with hydroxyl radical footprinting with RelB and RelBE. The core relB operator sequence was defined as WTGtWa. The association of RelB-E was probed by surface plasmon resonance (SPR) analysis.
The paper reports that TF forms complex with other proteins for binding with reported sites.
Binding is reported for RelB and for the complex RelBE.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|