Curation Information

Autoinducer binding by the quorum-sensing regulator TraR increases affinity for target promoters in vitro and decreases TraR turnover rates in whole cells.;Zhu J, Winans SC;Proceedings of the National Academy of Sciences of the United States of America 1999 Apr 27; 96(9):4832-7 [10220379]
TraR [P54294, view regulon]
Reported TF sp.
Agrobacterium tumefaciens R10
Reported site sp.
Agrobacterium tumefaciens R10
Created by
Joe Sparenberg
Curation notes

Experimental Process

EMSA showed binding of TraR to a predicted tra box binding site. DNase I footprinting confirmed that TraR precisely to the tra box. Site directed mutagenesis abolished detectable binding.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... traC traD traG Atu6123 traA traF traB traH
Experimental technique details DNAse footprinting (ECO:0005631) - Experimental technique details EMSA (ECO:0001807) - Experimental technique details In-vitro transcription (ECO:0001204) - not specified not specified