lacZ assays with promoter fragments of different lengths showed that TraR activates the expression of the repABC operon. EMSA showed direct binding to tra boxes 2 and 3. DNase I footprinting confirmed that the sequences were, in fact, tra boxes 2 and 3. Additionally, site-directed mutagenesis experiment validated the functionality of the tra box III.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|