Curation Information

Publication
A dual binding site for integration host factor and the response regulator CtrA inside the Caulobacter crescentus replication origin.;Siam R, Brassinga AK, Marczynski GT;Journal of bacteriology 2003 Sep; 185(18):5563-72 [12949109]
TF
CtrA [P0CAW8, view regulon]
Reported TF sp.
Caulobacter crescentus NA1000
Reported site sp.
Caulobacter crescentus NA1000
Created by
Dinara Sagitova
Curation notes
-

Experimental Process

DNase I footprinting showed that CtrA protected a region in the Cori probe containing a CtrA consensus (TTAA-N7-TTAA). Mutagenesis of the right TTAA motif resulted in the abolishment of binding by CtrA as observed by DNase I footprinting.

Transcription Factor Binding Sites


TTAACGCTCTGTTAA
TTAACGCTCTGTTAA

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
TTAACGCTCTGTTAA
... ... hemE hemH CCNA_03877
Experimental technique details DNAse footprinting (ECO:0005631) - Experimental technique details Site directed mutagenesis (ECO:0005667) - not specified not specified