Curation Information

Publication

Reconstitution of the trimethylamine oxide reductase regulatory elements of Shewanella oneidensis in Escherichia coli.;Gon S, Patte JC, Dos Santos JP, Méjean V;Journal of bacteriology 2002 Mar; 184(5):1262-9 [11844754]

TF

TorR [Q8EHJ3, view regulon]

Reported TF sp.

Shewanella oneidensis MR-1

Reported site sp.

Shewanella oneidensis MR-1

Created by

Dinara Sagitova

Curation notes

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Experimental Process

torECAD transcription start site was identified by primer extension analysis using RNA prepared from cells grown anaerobically with TMAO. torEC-lacZ fusion assays verified TorR-dependent regulation of torECAD. EMSA confirmed that this regulation is direct. DNase I footprinting localized the TorR binding site in the torECAD promoter. Deletion of the TorR box resulted in reduced expression and loss of binding.

Transcription Factor Binding Sites

• Sites reported in the paper
• Sites matched in the genome

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.