Curation Information

Reconstitution of the trimethylamine oxide reductase regulatory elements of Shewanella oneidensis in Escherichia coli.;Gon S, Patte JC, Dos Santos JP, Méjean V;Journal of bacteriology 2002 Mar; 184(5):1262-9 [11844754]
TorR [Q8EHJ3, view regulon]
Reported TF sp.
Shewanella oneidensis MR-1
Reported site sp.
Shewanella oneidensis MR-1
Created by
Dinara Sagitova
Curation notes

Experimental Process

torECAD transcription start site was identified by primer extension analysis using RNA prepared from cells grown anaerobically with TMAO. torEC-lacZ fusion assays verified TorR-dependent regulation of torECAD. EMSA confirmed that this regulation is direct. DNase I footprinting localized the TorR binding site in the torECAD promoter. Deletion of the TorR box resulted in reduced expression and loss of binding.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type