Curation Information

Publication
Managing oxidative stresses in Shewanella oneidensis: intertwined roles of the OxyR and OhrR regulons.;Li N, Luo Q, Jiang Y, Wu G, Gao H;Environmental microbiology 2014 Jun; 16(6):1821-34 [25009841]
TF
OhrR [Q8EI70, view regulon]
Reported TF sp.
Shewanella oneidensis MR-1
Reported site sp.
Shewanella oneidensis MR-1
Created by
Dinara Sagitova
Curation notes
Add bacterial one hybrid to the list of the techniques.

Experimental Process

qRT-PCR with mRNA from the wild-type and ohrR mutant S.oneidensis strains showed that ohr expression is repressed by OhrR in the absenses of tBOOH. Bacterial one-hybrid analysis confirmed that OhrR directly interacts with the ohr-ohrR intergenic region.

Transcription Factor Binding Sites


AATTAGATTGCGTGCAATCTAAAT
AATTAGATTGCGTGCAATCTAATT

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
AATTAGATTGCGTGCAATCTAATT ohrA, ohrR,
... ... ohrA ohrR glpD
Experimental technique details qRT-PCR [RNA] (ECO:0001808) - repressor not specified