Curation Information

Publication
AlgR functions in algC expression and virulence in Pseudomonas syringae pv. syringae.;Peñaloza-Vázquez A, Fakhr MK, Bailey AM, Bender CL;Microbiology (Reading, England) 2004 Aug; 150(Pt 8):2727-37 [15289569]
TF
AlgR [Q500P4, view regulon]
Reported TF sp.
Pseudomonas syringae pv. syringae strain FF5
Reported site sp.
Pseudomonas syringae pv. syringae strain FF5
Created by
Grace Chandler
Curation notes
-

Experimental Process

Visual inspection identified two putative AlgR binding sites resembling an AlgR consensus sequence. A GUS assays showed that functional copies of algR and rpoN were required for algC activation since its expression was reduced 4-fold in the AlgR mutant strain. EMSA confirmed that AlgR binds to the algC promoter.

Transcription Factor Binding Sites


CCGTCCGTGATGCT
CCGCTGTTTCAAGA
CCGTCCGTGATGCT
CCGCTGTTTCAAGA

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
CCGTCCGTGATGCT Psyr_0219,
... ... Psyr_0219 Psyr_0218
Experimental technique details EMSA (ECO:0001807) - Experimental technique details GUS reporter gene assay (ECO:0005641) - Experimental technique details Visual sequence inspection (nan) - activator not specified
CCGCTGTTTCAAGA Psyr_0219,
... ... Psyr_0219 Psyr_0218
Experimental technique details EMSA (ECO:0001807) - Experimental technique details GUS reporter gene assay (ECO:0005641) - Experimental technique details Visual sequence inspection (nan) - activator not specified