Using beta-gal reporter assays on the flaA promoter with different promoter fragment sizes, a region mediating temperature-dependent repression was identified. MogR was identified as a binding protein to the flaA promoter using affinity purification with magnetic beads and SDS-PAGE. Deletion of mogR was shown to increase flaA expression with beta-gal assays.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|