Curation Information

Publication

Listeria monocytogenes regulates flagellar motility gene expression through MogR, a transcriptional repressor required for virulence.;Gründling A, Burrack LS, Bouwer HG, Higgins DE;Proceedings of the National Academy of Sciences of the United States of America 2004 Aug 17; 101(33):12318-23 [15302931]

TF

MogR [P0DJO8, view regulon]

Reported TF sp.

Listeria monocytogenes EGD-e

Reported site sp.

Listeria monocytogenes EGD-e

Created by

Erill Lab

Curation notes

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Experimental Process

Using beta-gal reporter assays on the flaA promoter with different promoter fragment sizes, a region mediating temperature-dependent repression was identified. MogR was identified as a binding protein to the flaA promoter using affinity purification with magnetic beads and SDS
-PAGE. Deletion of mogR was shown to increase flaA expression with beta-gal assays.

Transcription Factor Binding Sites

• Sites reported in the paper
• Sites matched in the genome

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.