Primer extension analysis of the flaA promoter with RNA from wild-type and flrC, rpoN mutant V. cholerae strains determined the transcription start site of these genes. lacZ reporter assays with flaA promoter fragments of different lengths localized FlrC binding region to -54 to +66 bp relative to the TSS. EMSA confirmed that FlrC binds specifically to this promoter. DNase I footprinting localized the exact region bound by FlrC.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|