Site information for: PcaR in species Pseudomonas putida; genome accession: NC

Transcription Factor Binding Site Information

dbxref: CollecTF:EXPSITE_0000aeb0
Genome: Pseudomonas putida - NC_002947.3
TF: PcaR [UniProtKB:Q88N41, view regulon]

Supporting Evidence

Binding site	Location	Publication	Experimental techniques used	Curation
GTTCGATAATCGCAC	+ [4457196, 4457210]	8376330	Experimental technique details Beta-gal reporter assay Beta-gal reporter assay Reporter assay using the beta-galactosidase (lacZ) gene. The lacZ gene is typically fused to the promoter of interest. Differential regulation of the promoter mediated by the TF is assessed by induction of the system and evaluation of lacZ expression. Bacteria expressing lacZ appear blue when grown on a X-gal medium. The assay is often performed using a plasmid borne construction on a lacZ(def) strain. -	490

Regulated genes

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Gene	Locus tag	Description
pcaI	PP_3951	3-oxoadipate CoA-transferase subunit A
pcaJ	PP_3952	3-oxoadipate CoA-transferase subunit B
PP_3950	PP_3950	methyl-accepting chemotaxis transducer